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. 2018 May 4;9:1735. doi: 10.1038/s41467-018-03908-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — HP1a is required for the repression of selective transposons. a Representative images showing control and HP1a-GLKD fly ovaries stained for HP1a and HeT-A Gag proteins. Scale bars = 25 μm; n > 5. b Scatterplot showing the fold up-regulation of normalised RNA-seq reads mapping to canonical transposons in HP1a-GLKD compared to control ovaries. Telomeric transposons HeT-A, TART, and TAHRE are labelled in red. c Boxplot representing fold up-regulation in normalised RNA-seq reads mapping to telomeric and non-telomeric transposons in HP1a-GLKD vs. control. The middle line represents median. Box represents 25–75 percentile range called inter quartile range (IQR). Upper and lower whisker extend highest or lowest values till 1.5*IQR. Values above and below are outliers and plotted individually. d Ovaries with an HP1a mitotic clone (arrowhead) generated by FRT/FLP recombination stained for HP1a, GFP, and HeT-A Gag proteins. HP1a-null cells recapitulate show HeT-A upregulation. Scale bars = 25 μm; n = 3