Fig. 3.

HP1a represses Het-A in a location-dependent manner a Schematic representation of the HeT-A/lacZ reporter construct. The HeT-A promoter is fused to the lacZ gene. b Schematic representation of the HeT-A/lacZ reporter insertion at the cytological position 66C (H18). c Representative confocal images of HP1a, LacZ, and HeT-A immunostaining in tej^48-5/CyO, tej^48-5, and HP1a-GLKD ovaries. All genotypes harbour the HeT-A/lazZ transgene (H18 line). Three independent experiments were performed. Scale bars = 25 μm. d Bar graph showing the percentage of ovarioles expressing LacZ in tej^48-5/CyO, tej^48-5, and HP1a-GLKD genotypes bearing the HeT-A/lacZ transgene (H18 and H111). LacZ positive and negative ovarioles are represented by black and grey, respectively. n represents the number of ovarioles counted (n ≥ 100, from three independent experiments). e Representative confocal images of LacZ and HP1a immunostaining in the mitotic clones lacking HP1a. Red arrows and white arrows denote the HP1a clones with LacZ and those without LacZ expression, respectively. Scale bars = 15 μm. Three biological replicates were examined. f Scatterplot showing the mean likelihood of HP1a enrichment on chromosomal arms. Cytolocations marking telomeres and centromeres are represented in red. g UCSC browser screenshot showing the changes in total piRNAs and HP1a enrichment, following the normalisation with reads in the IgG ChIP-seq library, at cluster 42AB (upper panel) and cluster 38C (lower panel) in the control and HP1a-GLKD ovaries