Figure 7.

Biomarkers of inflammation increased in response to allergen challenge. Cytokines in the bronchoalveolar lavage fluid were measured in each genotype and found to be elevated in mice subjected to the fungal asthma model. Notably, Retnlb deficient mice had elevated TNFα, VEGF, and IFNγ at steady state compared to WT controls (A). Wild-type mice had rapid increase in cytokines at early time points with gradual depletion over time whereas Retnlb KO followed similar trends in cytokine production, albeit at slightly lower levels at early time points (A). Wild-type mice had markedly elevated expression of Retnla and SurfA in the lung while Retnlb null mice had markedly elevated Camp and Il13 (B). Expression of Tlr2 dominated at the early time point in the wild-type mice while Trl4 took precedence in the Retnlb null mice (B). Data shown as range from minimum to maximum where lines and dots represent the median and mean respectively (A) and as the mean and SD (B) of n = 4–7 mice/group and representative of one independent study of two. Data analysed with two-way ANOVA with Sidak’s and Dunnett’s multiple comparisons test to compare data between groups at each time point (*) or to naïve controls within groups († in WT and Δ in KO) respectively, where significance values p < 0.05, p < 0.01, and p < 0.001 are denoted by one, two, or three symbols respectively. N – Naïve.