Figure 7.

Addition of a bulky tag on TDP-43 disrupts passive but not active nuclear import. (a) Domains maps (to scale) of WT TDP-43 containing synonymous mutations which confer resistance to TDP-43 siRNA (indicated by asterisk), Flag WT TDP-43, tdTomato TDP-43. Formatted using IBS cuckoo⁴³. (b) Immunofluorescence of HeLa cells expressing the indicated protein. Selected samples were treated with TDP-43 siRNA to deplete endogenous TDP-43. Resistant TDP-43 was visualized via immunofluorescence with TDP-43 antibody; Flag TDP-43 was visualized via immunofluorescence with Flag antibody; tdTomato TDP-43 was visualized with direct fluorescence. Nuclei were visualized using Hoechst stain. Scale bar- 10 um. Images are representative of 3 independent experiments.