Table 1.

Solvent accessibility surface of residues within TDP-43 putative NES.

Amino Acid	Residue #	Ratio (%)	In/Out
ILE	239	16.5	i
ALA	240	0	i
GLN	241	39.1
SER	242	78.5	o
LEU	243	6.3	i
CYS	244	4.1	i
GLY	245	13.5	i
GLU	246	56.4	o
ASP	247	3.6	i
LEU	248	7.8	i
ILE	249	25.5
ILE	250	0.1	i

Using the NMR data from Lukavsky et al.²⁰ and an algorithm developed by Braun et al.⁴⁴, the solvent accessibility of the putative NES was calculate (hydrophobic residues bolded). The first two columns are the amino acid identity and residue number. The third is the side chain surface area, normalized to a “random coil” of that residue. Resides are classified as solvent exposed if this normalized value exceeds 50% (labelled “o”) and buried if the normalized value is less than 20% (labelled “i”).