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. 2018 May 4;8:7055. doi: 10.1038/s41598-018-25489-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Regulatory effects of miR-143 on the expression of NPPA, NPPC, NR3C2 and CRHR2. (a) Luciferase reporter assay to demonstrate the interactions between miR-143 and the 3′UTRs of NPPA, NPPC, NR3C2 and CRHR2. (b) Overexpression of miR-143 (miR-143 mimic) suppressed expression of NPPA, NPPC, and NR3C2 in HCMa cells. However, knockdown of NPR3 level with antagomiR-143 did not lead to enhancement of NPPA, NPPC, and NR3C2 levels in HCMa cells. Data are presented as mean ± SD, n = 3, Student’s t test, *p < 0.05.