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. 2018 May 4;9:1800. doi: 10.1038/s41467-018-04238-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Prevention of Aβ accumulation in 6-month-old App 3′-UTR deleted NL-G-F mice. a Aβ pathology in 6-month-old NL-G-F ΔUTR mouse brains, as well as unedited control NL-G-F brains. Scale bar: 1 mm. b Negative correlation between Aβ accumulation and genome editing efficiency in NL-G-F ΔUTR mouse brains (n = 13). c Positive correlation between normalized APP protein expression and genome editing efficiency in NL-G-F ΔUTR mouse brains (n = 13). Western blotting was performed using an antibody against N terminus of APP protein. d–g Negative correlation between normalized Aβ levels and deletion efficiency in 6-month-old NL-G-F ΔUTR mouse brains (n = 13). Aβ₄₀ and Aβ₄₂ levels in both the Tris-HCl-buffered saline (TS) and GuHCl fractions were quantified by ELISA. h Strategy of CRISPR/Cas9-mediated NL-G-F Δ400. Positions of targeting sgRNAs are shown by scissors. i Negative correlation between Aβ levels and deletion efficiency in female NL-G-F Δ400 mouse brains (n = 5). j Positive correlation between normalized APP protein expression and genome editing efficiency in NL-G-F Δ400 mouse brains (n = 11)