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. 2018 May 4;8:7062. doi: 10.1038/s41598-018-25571-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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HMPC rescues C. elegans from MRSA infection. (A) Chemical structure of propyl 5-hydroxy-3-methyl-1-phenyl-1H-pyrazole-4-carbodithioate (HMPC). (B–D) Results of compound screening for C. elegans viability following S. aureus MW2 infection. A 384-well assay plate was co-inoculated with C. elegans, S. aureus MW2 and either 7 μg/ml HMPC (B); 100% survival), 10 μg/ml vancomycin (C) positive control; 100% survival), or 1% DMSO vehicle (D) negative control; 40% survival). Representative Sytox Orange-stained (right) and bright field (left) images from assay wells are shown. Only dead worms take up Sytox Orange and fluoresce. The images were first captured with an ImageExpress and further processed with CellProfiler to normalize the fluorescent or bright field intensity and to define the area of each worm.