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. 2018 May 4;8:7062. doi: 10.1038/s41598-018-25571-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Mammalian cell toxicity assays. (A) HMPC hemolysis assay. Red blood cells were diluted to 2% with PBS and then incubated with HMPC over the concentration range 0.125−64 μg/ml. (B) Cytotoxicity of HMPC towards HKC-8 cells. HKC-8 cells were treated with increasing concentrations of HMPC for 24 hours before assessing viability using a WST-1 assay. (C) Cytotoxicity of HMPC towards HepG2 cells. HepG2 cells were treated with increasing concentrations of HMPC for 24 hours before assessing viability using a WST-1 assay. All experiments were repeated twice in triplicate and data show the mean +/− standard deviation.