Table 3.
Mutations identified in HMPC-resistant S. aureus mutants selected in vitro.
| Selection | MIC (μg/ml) | MW2 pos (bp) | Typea | Length (bp) | Gene ID | Frequency (%) | Protein changeb |
|---|---|---|---|---|---|---|---|
| 1 | 8 | 731157 | MNV | 17 | MgrA | 46.8 | G108fs |
| 731244 | SNV | 1 | MgrA | 51.9 | R84H | ||
| 2 | 8 | 731332 | SNV | 1 | MgrA | 100 | V55F |
| 3 | 8 | 731158 | Deletion | 12 | MgrA | 52.9 | L109del4 |
| 731283 | SNV | 1 | MgrA | 28.9 | P71N | ||
| 731470 | SNV | 1 | MgrA | 10.5 | E9* | ||
| 1 | 16 | 731244 | SNV | 1 | MgrA | 100 | R84H |
| 1366975 | Deletion | 4 | FmtC | 93.5 | I461fs | ||
| 2 | 16 | 731295 | SNV | 1 | MgrA | 20.9 | G67A |
| 731332 | SNV | 1 | MgrA | 77.4 | V55F | ||
| 1366139 | Deletion | 7 | FmtC | 28 | I181fs | ||
| 3 | 16 | 731283 | SNV | 1 | MgrA | 100 | P71N |
| 1005808 | Insertion | 1 | Hyp YdiL | 85 | L151fs | ||
| 1366760 | Deletion | 684 | FmtC | 100 | N386del228 |
aMNV = Multi-nucleotide variant; SNV = Single Nucleotide Variant.
bfs = frame-shift; *stop codon.