Figure 3.

Expression levels and mutation status of MET and EGFR in relevant cell lines and xenografts. (a) western blot of cell lines E98, Hs746T and H596, U87-EV, U87-EGFRvIII, and xenografts E98-FM and E468, showing relative (total) MET and EGFR expression. Note that MET shows two proteins of different sizes, corresponding to the preform and the processed form of the protein⁸. (b) Left: MET PCR on cell lines E98, Hs746T and H596 to confirm MET^Δ7-8 and MET^Δ14 mutation status. Right: EGFR PCR on cell lines U87-EV and U87-EGFRvIII, and xenograft E468, to confirm EGFR^wt and EGFRvIII status. (c) SmMIP targeted transcriptome profiling of MET (left) and EGFR (right) quantifies variant-specific expression levels in cell lines and xenografts. While E98 cells and xenografts express the MET^Δ7-8 variant, Hs746T and H596 predominantly express the MET^Δ14 variant. U87 cells express MET^wt transcripts. EGFR^wt transcript expression is present in Hs746T, H596, U87 and E468 cells and xenografts, while the EGFRvIII variant is only present in the U87-EGFRvIII cell line.