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. 2018 May 4;8:7072. doi: 10.1038/s41598-018-25328-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Multi-plex in situ mutation detection of MET using padlock probe rolling circle amplification on cell lines. In situ duo-probe detection of METexon7–8^wt and MET^Δ7-8 transcripts in E98 cells (left) and METexon14^wt and MET^Δ14 transcripts in Hs746T cells (right) using padlock probes MET^7,8 and MET^6–9, and MET¹⁴ and MET^13–15, respectively. METexon7–8^wt and METexon14^wt transcripts are displayed as green RCPs, MET^Δ7-8 and MET^Δ14 transcripts are displayed as magenta RCPs. Cell nuclei are shown in grey. Original magnification 40x.