Figure 7.

Multi-plex in situ mutation detection of EGFR using padlock probe rolling circle amplification on cell lines and E468 xenograft tissue. A) In situ duo-probe detection of EGFR^wt and EGFRvIII transcripts using padlock probes EGFR^2,3 and EGFR^1–8, on U87-EV and U87-EGFRvIII cells (original magnification 40x). B) In situ duo-probe detection of EGFR^wt and EGFRvIII transcripts using padlock probes EGFR^2,3 and EGFR^1–8, on E468 xenograft FFPE tissue (original magnification 20x). EGFR^wt transcripts are displayed as green RCPs, EGFRvIII transcripts are displayed as magenta RCPs. Cell nuclei are shown in grey.