Table 1.
SmMIP nucleotide sequences. Ligation and extension probes are depicted in Italic.
| Transcript | Exon targets | MIP sequence (5′→3′) | |
|---|---|---|---|
| Extension | Ligation | ||
| variant | probe | probe | |
| Total MET | Exon 9 | Exon 9–10 | GTGGTGGGAGCACAATAACAGGTGTTGNNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTCAAACCATTTCAACTGAG |
| (variant 2) | Exon 9 | Exon 11 | GCATGTCAACATCGCTCTAATTCAGNNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTATTCATCCAACCAAATCTTT |
| METΔ7-8 | Exon 6 | Exon 6–9 | GCACGATGAATACTGTGTCAAACAGNNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTTTGAAGGAGGGACAAGGCTG |
| Exon 6 | Exon 9 | GCCAACCGAGAGACAAGCATCTTCANNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTAATGAGAGCTGCACCTTGAC | |
| METΔ14 | Exon 13 | Exon 15 | GTTTCCTAATTCATCTCAGAACGGTTCANNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTAATAGTTCAACCAGATC |
| Exon 13 | Exon 15 | CAGTCCATTACTGCAAAATACTGTCCACANNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTAAAAAGAGAAAGCAAA | |
| Total EGFR | Exon 1 | Exon 2 | GGAAATTACCTATGTGCAGAGGAATTATGNNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTCTGGAGGAAAAGAAAG |
| Exon 3 | Exon 3 | GCAAATAAAACCGGACTGAAGGAGCNNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTGTGGCTGGTTATGTCCTCAT | |
| Exon 3 | Exon 4 | GCCCTGTGCAACGTGGAGAGCATCNNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTCTACGAAAATTCCTATGCCTT | |
| Exon 5–6 | Exon 6–7 | GCCTGGTCTGCCGCAAATTCCGAGACGAANNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTCCAGAAACTGACCAAA | |
| Exon 7 | Exon 8 | GCCTGTGGGGCCGACAGCTATGAGATGGANNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTTCTACAACCCCACCAC | |
| Exon 9 | Exon 10–11 | CGTAAAGGAAATCACAGGGTTTTTGCTGANNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTAAACACTTCAAAAACT | |
| Exon 15 | Exon 17 | GCCCTGGGGATCGGCCTCTTCATNNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTACCTGTGCCATCCAAACTGCAC | |
| Exon 17–18 | Exon 18–19 | GCACGGTGTATAAGGGACTCTGGATNNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTTGCTGCAGGAGAGGGAGCTT | |
| Exon 18 | Exon 19–20 | GCCAACAAGGAAATCCTCGATGAAGCNNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTAAAAGATCAAAGTGCTGGG | |
| Exon 19 | Exon 20 | TCTGCCTCACCTCCACCGTGCANNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTAAGTTAAAATTCCCGTCGCTATC | |
| Exon 20 | Exon 20 | GCTCCCAGTACCTGCTCAACTGGTGTGTNNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTTGGACAACCCCCACGT | |
| Exon 21 | Exon 21–22 | GCAGAAGGAGGCAAAGTGCCTATCAANNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTAGGACCGTCGCTTGGTGCA | |
| Exon 21 | Exon 23 | GAGTTGATGACCTTTGGATCCAAGCCNNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTCGGAAGAGAAAGAATACCA | |
| Exon 23–24 | Exon 25 | GCCAAGTCCTACAGACTCCAACNNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTCATGGTCAAGTGCTGGATGATAG | |
| Exon 26 | Exon 27 | GACAGCATAGACGACACCTTCCTCCCAGNNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTAGTGCAACCAGCAACAA | |
| Exon 28 | Exon 28 | GCCACCAAATTAGCCTGGACAACCCTGNNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTAGAGACCCACACTACCA | |
| EGFRvIII | Exon 1 | Exon 8 | GTGGTGACAGATCACGGCTCGTGNNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTGAGAGCCGGAGCGAGCTCTT |
| Exon 1 | Exon 8–9 | GCCGCAAAGTGTGTAACGGAATAGGTANNNNNNNNCTTCAGCTTCCCGATATCCGACGGTAGTGTCTGGAGGAAAAGAAAG | |