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. 2018 May 4;9:1801. doi: 10.1038/s41467-018-04236-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Relative abundance of mcrA gene copies in amended sediments. Relative abundance is orders of magnitude higher in sediments amended with emergent macrophyte (Typha latifolia; TYP) litter than deciduous (DEC) or coniferous (CON) forest litter and mirrors CH₄ production in Fig. 1. DNA was pooled across replicates (n = 4 per %OM treatment) and expressed as relative abundance per gram dry-weight (g_dw) of sediment normalized for extraction yield determined by qPCR. Samples were run in triplicate and compared to a standard curve generated from eDNA PCR product to capture the environmental variability in sequences. Error bars for amendments represent standard error across %OM treatments (10, 20, 40%)