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. 2018 May 4;8:7044. doi: 10.1038/s41598-018-25395-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression and morphological analysis of human OA cartilage-derived stem cell lines. (A) Quantification of CD49e and COL2A1 mRNA levels in primary human osteoarthritis mesenchymal stem cells (OA-MSC), compared with primary human chondrocytes (PHC). (B) Relative aggrecan (ACAN), (C) type II collagen (COL2A1), (D) fibronectin receptor CD49e and (E) NOTCH1 mRNA expression levels in PHCs and nine OA-MSC lines. OA-MSC lines were categorized into two groups (GI, GII) based on their relative ACAN and COL2A1 expression levels. Due to the wide distribution of relative expression values, a logarithmic scale on the y-axis is implemented in B-D. (F) Cellular morphologies of OA-MSC2, OA-MSC18, PHCs and bone marrow derived mesenchymal stem cells (BM-MSCs). Images were acquired at an original magnification of 10× using an inverted microscope, Scale bar represents 100 µm. For all quantitative data, n ≥ 3. *p ≤ 0.05; **p ≤ 0.01, relative to PHCs. Quantitative data are represented as mean ± SD.