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. 2018 May 4;8:7085. doi: 10.1038/s41598-018-25349-0

Figure 3.

Figure 3

Alternative splicing event in HvSPL genes and expression analysis. (A) Number of Splice variants in HvSPL genes and miR156 target site distribution. (B,C) The splice variants of barley SPL11 and their expression pattern in 10, 16 and 75 day old plant. The two variants (SPL11V1 and V2) expressed differentially. (D,E) Splice variant of barley SPL8 and their expression patterns. The three variants (SPL8V1, V2 and V3) of SPL8 expressed lower in juvenile and higher in reproductive phase. (F,G) Two splice variant (SPL20V1 and V2) of barley SPL20 and their expression patterns. *Asterisks denote the presence of the miR156 complementary sequence in the splice variants of barley SPL11 gene. The brown colour box represents coding region, black line denotes intron and the white rectangle denotes 5′ & 3′ UTR regions. The arrow shows the site of forward and reverse primers. For more clarity gel area showing relevant bands were cropped. The full-length gels are presented in Supplementary Fig. S3.