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. 2018 May 4;8:7085. doi: 10.1038/s41598-018-25349-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of HvSPL genes during growth phase transition in barley. (A) Transcript abundance of HvSPL13 and (B) HvSPL6 at vegetative (10 days) and reproductive (75 days) phases. (C) Spike architecture of barley mir172 mutant and wild type GP. A single 3.6 kb Ds insertion in mir172 mutant was previously identified by Brown and Bregitzer, 2011. This mutant possesses abnormal spike at both apical region (glumes were changed to florets) and basal region (abnormal branched phenotype). (D–H) Expression of HvSPL3, 13, 15, 23 and AP2 genes in the spike of barley mir172 mutant and wild type GP. Transcript abundance was measured by qRT-PCR.