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. 2018 May 4;8:7076. doi: 10.1038/s41598-018-25470-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Michaelis-Menten analyses of GFP-CHs. Representative results of triplicates were shown for individual substrates. The initial rates of ClpXP-mediated proteolysis at various GFP-UCHs concentrations (as indicated below the X-axis of each panel) were extracted from fitting the changes in GFP fluorescence within the first 60 seconds.