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The enzymatic defect of sta11-1 mutant strains. Denatured crude extracts (100 μg of protein) from two wild-type (+) and three sta11-1 (−) were loaded on denaturing polyacrylamide gels. The proteins were renatured after electrophoresis and incubated overnight. Glc production was revealed after overnight incubation of the gel with 3 mg mL−1 maltotriose. The 62-kD blue-staining band can be easily distinguished in the wild-type cells. This band contained the D-enzyme activity.
