Fig. 6.

Effect of puerarin on the expressions of p-p38 MAPK and PCNA in VSMCs. Cells were pre-treated with puerarin at different concentrations (0, 12.5, 25, 50 μM) or p38 MAPK inhibitor SB203580 (20 μM) for 1 h and followed by the addition of 200 mg/L PM2.5 for 24 h. Then the expressions of PCNA, p-p38 MAPK and t-p38 MAPK in VSMCs were determined by western blot, β-actin was used for the protein loading control. a Representative western blot results for PCNA, p-p38 MAPK and t-p38 MAPK in VSMCs. b Quantitative analysis of western blot results was presented. The results are presented as mean ± SEM. n = 3. Compared to the untreated cells, ^*P < 0.05, ^**P < 0.01; compared to 200 mg/L PM2.5 group, ^#P < 0.05, ^##P < 0.01; compared to the co-incubation of puerarin with SB203580 group, ^▲P < 0.05, ^▲▲P < 0.01