Fig. 1.

Comparison of mRNA and protein expression in wild-type (WT) and transgenic plants. Empty, empty vector; AtCYO1ox, AtCYO1 overexpression vector. a Expression of AtCYO1 in transgenic lines relative to WT (mean ± SE; n = 3). b SDS-PAGE gel with Coomassie brilliant blue staining of photosynthetic proteins in crude extracts. RBC-L, large subunit of Rubisco; LHCP, light-harvesting complex protein. c Accumulation of AtCYO1 protein in cotyledons and rosette leaves. d Accumulation of AtCYO1 in pelleted chloroplasts and supernatants (Sup.) of rosette leaves. In b, c and d, the same amount of sample was loaded in all lanes (400 μg fresh weight in b; 50 μg protein in c, d). In a, b and d, samples were taken from 2-week-old plants. In c, samples were taken from 7-day-old seedlings for cotyledons and 4-week-old plants for rosette leaves