Fig. 5.

Proliferation inhibition and apoptosis induced by A35 are dependent on YAP phosphorylation (Ser127). HCT116 and HCT116-KO cells were treated with A35 for 24 h, and whole proteins were detected by immunoblot with indicated antibodies (a). Nuclear and cytoplasmic proteins were extracted and detected by immunoblot (b). YAP and YAPS127A constructs were transfected into YAP-HCT116^−/− cells, and after 24 h A35 was added for an additional 24 h to detect the indicated proteins (c) and for 48 h to detect cell survival by the SRB assay (d) and apoptosis by TUNEL assay (e). *P < 0.05; ** P < 0.01