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Kinetic constants of the StHK1 (expressed in the YSH7.4-3C yeast strain) for Glc, Fru, and Man
| Substrate | Km | Vmax | Phosphorylation Coefficient |
|---|---|---|---|
| mm | nmol min−1 mg−1 protein | ||
| Glc | 0.033 | 349 | 1.000 |
| Fru | 1.470 | 523 | 0.033 |
| Man | 0.029 | 221 | 0.710 |
The Km and Vmax values were calculated from Eadie-Hofstee plots. Each value is the mean of three independent assays (se was 2%–16% of the mean).
