Table II.
Hexokinase activity in antisense (αHK1) and overexpression (PHK1) transgenic lines
| HK Activity
|
||
|---|---|---|
| Line | Leaf | Tuber |
| nmol min−1 g−1 fresh wt | ||
| Antisense (αHK1) | ||
| Wild type | 61 ± 2 | 101 ± 4 |
| 8 | 25 ± 3 | 33 ± 1 |
| 9 | 29 ± 1 | 42 ± 2 |
| 11 | 19 ± 3 | 49 ± 6 |
| 13 | 13 ± 1 | 44 ± 7 |
| 48 | 26 ± 1 | 36 ± 5 |
| 60 | 23 ± 1 | 47 ± 5 |
| 82 | 19 ± 1 | 33 ± 4 |
| Overexpression (PHK1) | ||
| Wild type | 72 ± 3 | 106 ± 5 |
| 5 | 214 ± 14 | 166 ± 4 |
| 22 | 267 ± 22 | 154 ± 4 |
| 32 | 151 ± 11 | 130 ± 6 |
| 37 | 300 ± 37 | 174 ± 5 |
| 70 | 351 ± 26 | 188 ± 9 |
| 89 | 143 ± 9 | 137 ± 4 |
| 95 | 265 ± 15 | 222 ± 11 |
Plants were grown in the greenhouse in the autumn/winter season in 3.5-L pots. Leaf samples were taken from 8-week-old plants, tuber samples from 12-week-old plants. Data are presented as the means ± se; n = 6.