Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 May 4;11:273. doi: 10.1186/s13104-018-3378-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 1 — Stability of blood plasma miR-16-5p and miR-21-5p to freeze–thaw and room temperature incubation is associated with removal of platelets. Total RNA was isolated from PRP or PPP immediately after processing (fresh), after one through six freeze–thaw cycles of – 80 to 22 °C (FT 1–6), or after incubation of plasma at 22 °C for 24 h (RT 24). miR-16-5p and miR-21-5p levels as well as a synthetic cel-miR-39-3p spike-in (spike) were assessed by stem-loop/hydrolysis probe qPCR assays, with results presented as Cq. Data are average plus and minus standard deviation for processing replicates. Three processing replicates and three qPCR measurements are included for each condition. No RT and no template reactions were also performed, with all Cq > 37 or undetected (not shown)