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Culture of CMECs isolated from adult male Sprague-Dawley rat heart tissue (left ventricle). (A) Primary isolated rat CMECs (100×). (B) Rat CMECs at passage 2 (100×). (C) Immunostaining for CD34 in rat CMECs (100×). (D) Immunostaining for von Willebrand factor in rat CMECs (200×).