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Effect of 17β-estradiol (17β-E2) on rat CMEC proliferation. (A) Cell cultures were treated with the indicated concentration of 17β-E2, and cell proliferation was quantified using the MTT assay. * P<0.05 vs. control, *** P<0.001 vs. control; n=6. (B) Tamoxifen inhibition of 17β-E2-induced cell proliferation. Cell cultures were untreated or treated with 17β-E2 (0.01 μmol/l) in the absence or presence of tamoxifen (Tamo; 0.1 μmol/l) *** P<0.001 vs. control; n=6.
