Fig. 1.

Discrete enhancer and promoter activities of Actβ, Daw, and Myo genes in adult fruit flies. Each of the three transgenic fruit fly lines, Actβ-Gal4, Daw-Gal4, and Myo-Gal4, was crossed either to a UAS-GFP reporter line or to a UAS-RedStinger line. The green fluorescence protein (GFP) signal or RedStinger signal from the progeny of those three crosses was monitored by a confocal microscope. The dissected adult fly brains from Daw-Gal4/UAS-RedStinger or Myo-Gal4/UAS-RedStinger were stained by an anti-Repo antibody for glial cells. (A,B) Confocal pictures showing GFP expression from brain tissues (A) and motor neurons (B) of a 1-week-old adult fruit fly with a genotype Actβ-Gal4/UAS-GFP. (C) Daw-expressing glial cells (red) from a 1-week-old adult fly brain that was co-stained by an anti-Repo antibody (green). (D,E,F) Strong GFP expression was observed in muscle (D), adipose tissues (E), and the gut (F) of a 1-week-old Daw-Gal4/UAS-GFP fruit fly. (G) Myo-Gal4 driven RedStinger expression was observed in glial cells of adult fruit fly brain that was stained by an anti-Repo antibody (green). (H) Despite the strong expression of Myo in larval body wall muscle tissues, we could not see strong GFP signal from the muscle tissues of a 1-week-old Myo-Gal4/UAS-GFP fruit fly. (I) Strong GFP signals were also detected in the gut tissues of a 1-week-old Myo-Gal4/UAS-GFP fruit fly.