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. 2018 Apr 15;7(4):bio030361. doi: 10.1242/bio.030361

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© 2018. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Deletion of the PH4 gene cluster at 75D inhibits efficient higher-order multimerisation of Pericardin. (A,B) Protein extracts isolated from homozygous animals with the genotype Df(3L)Exel6134 (deleting the PH4 gene cluster at 75D) (A) and Df(3R)Exel6215 (deleting the PH4 gene cluster at 99F) (B) were probed for anti-Pericardin under oxidising and reducing conditions. As loading control Western blots were probed for GAPDH or membranes were stained for Ponceau. (C) Wild-type, homozygous Df(3L)Exel6134 and Df(3R)Exel6215 and homozygous garzΔ¹³⁷ mutant embryos (stg. 16/17) were stained with anti-Prc.