Fig. 6.

The effect of JHM given at pupariation on EcR-B1 and Br-Z3 expression in the lamina during adult development. The JHM pyriproxifen was applied at pupariation (P0) and images taken at 12 and 24 h thereafter of both the JHM-treated and the untreated controls. The control is also shown at 46 h APF. The top composite images show the pan-lamina line R27G05-GAL4 driving a myristylated GFP reporter in all the monopolar lamina neurons (L1-L5) and immunocytochemical staining for both EcR-B1 (red) and Br-Z3 (blue). The middle and bottom rows show the gray scale images of the Broad (Br-Z3) and EcR-B1 immunostaining respectively. Images are typical of three to four control animals and five JHM-treated animals for each time point. The images are sections viewed along the dorsoventral axis. The lamina neuron cell body region is indicated by the yellow dashed line. Scale bars: 50 µm (the one in the Control P0 column pertains to only that column; the one in the Control P+46 h column pertains to the remainder of the images).