Table 1. BOLD distance analysis of the studied Gerromorpha and Nepomorpha.
| Family | Species | n | PC | BIN | MID | DNN | NNS |
|---|---|---|---|---|---|---|---|
| Aphelocheiridae | Aphelocheirus aestivalis | 2 | Mono | ABX0398 | 0 | 11.86 | Notonecta maculata |
| Corixidae | Arctocorisa carinata | 5 | Para | AAJ7903, ACY1261 | 2.36 | 1.03 | Arctocorisa germari |
| Arctocorisa germari | 1 | n. a. | – | 0 | 1.03 | Arctocorisa carinata | |
| Callicorixa praeusta | 23 | Para | AAK1938 | 0.31 | 0 | Callicorixa producta | |
| Callicorixa producta | 1 | n. a. | AAK1938 | 0 | 0 | Callicorixa praeusta | |
| Corixa affinis | 13 | Mono | ACY0615 | 1.92 | 5.92 | Corixa panzeri | |
| Corixa dentipes | 1 | n. a. | – | 0 | 6.08 | Corixa punctata | |
| Corixa panzeri | 2 | Mono | ACX9506 | 0 | 5.92 | Corixa affinis | |
| Corixa punctata | 21 | Mono | ACB1799 | 0.77 | 6.08 | Corixa dentipes | |
| Cymatia bonsdorffii | 4 | Mono | ABX0396 | 0.62 | 12.34 | Cymatia coleoptrata | |
| Cymatia coleoptrata | 24 | Mono | ACB1796, ADD1561 | 9.44 | 12.4 | Cymatia bonsdorffii | |
| Cymatia rogenhoferi | 1 | n. a. | ACB2132 | 0 | 12.7 | Cymatia coleoptrata | |
| Glaenocorisa propinqua | 8 | Mono | ABX4248 | 1.55 | 9.96 | Sigara semistriata | |
| Hesperocorixa castanea | 14 | Mono | ABX0447 | 0.32 | 13.22 | Paracorixa concinna | |
| Hesperocorixa linnaei | 8 | Mono | ABX0448 | 0 | 11.89 | Sigara venusta | |
| Hesperocorixa sahlbergi | 39 | Mono | AAN0795 | 1.7 | 11.34 | Corixa panzeri | |
| Micronecta griseola | 2 | Mono | AAK6480 | 0 | 10.63 | Micronecta poweri | |
| Micronecta poweri | 2 | Mono | ACB1970 | 2.39 | 10.63 | Micronecta griseola | |
| Micronecta scholtzi | 6 | Mono | AAK6479 | 0.16 | 18.58 | Sigara semistriata | |
| Paracorixa concinna | 11 | Mono | ABV3365, ADG5371 | 1.71 | 7.03 | Sigara semistriata | |
| Sigara distincta | 7 | Poly | ABY7152, ABV4484 | 5.77 | 0.37 | Sigara falleni | |
| Sigara dorsalis* | 1 | n. a. | AAJ6688 | 0 | 1.71 | Sigara striata | |
| Sigara falleni | 12 | Poly | AAH9524, ABY7152 | 3.37 | 0 | Sigara iactans | |
| Sigara fossarum | 3 | Mono | AAJ6707, ADD1512 | 2.82 | 2.72 | Sigara scotti | |
| Sigara hellensii | 2 | Mono | ADH9592, ACT7694 | 4.41 | 9.09 | Sigara distincta | |
| Sigara iactans | 12 | Poly | ABY7152, AAH9524 | 2.67 | 0 | Sigara falleni | |
| Sigara lateralis | 14 | Mono | AAJ6697 | 0.81 | 9.84 | Sigara striata | |
| Sigara limitata | 2 | Para | ACM1221 | 0.48 | 0.15 | Sigara semistriata | |
| Sigara nigrolineata | 16 | Mono | ACB1978 | 0.46 | 10.12 | Sigara semistriata | |
| Sigara scotti | 12 | Mono | ACY0807 | 1.08 | 2.72 | Sigara fossarum | |
| Sigara semistriata | 5 | Poly | ACM1221 | 0 | 0.15 | Sigara limitata | |
| Sigara stagnalis | 6 | Mono | ACY0713 | 0.55 | 11.45 | Paracorixa concinna | |
| Sigara striata | 10 | Mono | AAJ6688 | 0.93 | 1.71 | Sigara dorsalis | |
| Sigara venusta | 2 | Mono | ABA5309 | 0 | 2.11 | Sigara semistriata | |
| Naucoridae | Ilyocoris cimicoides | 17 | Mono | AAF2590 | 1.03 | 15.06 | Hesperocorixa sahlbergi |
| Nepidae | Nepa cinerea | 10 | Mono | AAK8359 | 0.34 | 17.06 | Notonecta maculata |
| Ranatra linearis | 16 | Mono | AAL1328 | 0.84 | 15.03 | Notonecta lutea | |
| Notonectidae | Anisops sardeus* | 5 | Mono | ABV0079 | 1.24 | 12.84 | Notonecta maculata |
| Notonecta glauca | 41 | Mono | AAK4442 | 1.71 | 1.08 | Notonecta obliqua | |
| Notonecta lutea | 19 | Mono | AAN1701 | 0.68 | 1.24 | Notonecta reuteri | |
| Notonecta maculata | 10 | Mono | AAN1703 | 2.43 | 6.56 | Notonecta glauca | |
| Notonecta obliqua | 9 | Mono | AAK4442 | 0.64 | 1.08 | Notonecta glauca | |
| Notonecta reuteri | 5 | Mono | ACE8526 | 0.46 | 1.24 | Notonecta lutea | |
| Notonecta viridis | 10 | Mono | ABV0133 | 1.18 | 5.03 | Notonecta glauca | |
| Pleidae | Plea minutissima | 17 | Mono | ACY0868, AAF3832 | 8.3 | 10.92 | Notonecta lutea |
| Gerridae | Aquarius najas | 7 | Mono | AAN1521 | 2.14 | 11.75 | Gerris thoracicus |
| Aquarius paludum | 19 | Mono | AAI7450 | 1.24 | 12.61 | Gerris argentatus | |
| Gerris argentatus | 32 | Mono | ADD1846 | 0.72 | 6.55 | Gerris odontogaster | |
| Gerris asper | 1 | n. a. | ABA3327 | 0 | 0.34 | Gerris lateralis | |
| Gerris costae | 11 | Mono | ACI6181 | 0 | 7.48 | Gerris thoracicus | |
| Gerris gibbifer | 11 | Mono | ACB1756 | 0.88 | 8.91 | Gerris lacustris | |
| Gerris lacustris | 38 | Mono | ACT3584 | 1.05 | 8.91 | Gerris gibbifer | |
| Gerris lateralis | 2 | Mono | ABA3327 | 0.17 | 0.34 | Gerris asper | |
| Gerris odontogaster | 19 | Mono | ABU6679, ADD1838 | 1.59 | 6.55 | Gerris argentatus | |
| Gerris thoracicus | 6 | Mono | ACB1745 | 0.35 | 7.48 | Gerris costae | |
| Limnoporus rufoscutellatus | 3 | Mono | AAV0261 | 0.88 | 11.86 | Gerris asper | |
| Hebridae | Hebrus pusillus | 2 | Mono | AAN0981 | 0.15 | 14.32 | Hebrus ruficeps |
| Hebrus ruficeps | 7 | Mono | AAI6967 | 0.15 | 14.32 | Hebrus pusillus | |
| Hydrometridae | Hydrometra gracilenta | 9 | Mono | AAN0857 | 0.46 | 13.06 | Hydrometra stagnorum |
| Hydrometra stagnorum | 21 | Mono | AAK5632 | 0.62 | 13.06 | Hydrometra gracilenta | |
| Mesoveliidae | Mesovelia furcata | 17 | Mono | AAN2451 | 1.39 | 16.24 | Mesovelia vittigera |
| Mesovelia vittigera* | 4 | Mono | ACD4048 | 2.32 | 16.24 | Mesovelia furcata | |
| Veliidae | Microvelia buenoi | 1 | n. a. | ACY1789 | 0 | 15.06 | Gerris costae |
| Microvelia reticulata | 27 | Mono | AAG4341 | 0.77 | 15.04 | Gerris asper | |
| Velia caprai | 20 | Mono | AAN0455 | 1.1 | 4.94 | Velia saulii | |
| Velia currens* | 3 | Mono | ADI1962 | 0 | 2.82 | Velia saulii | |
| Velia saulii | 1 | n. a. | ABX0836 | 0 | 2.82 | Velia currens |
Notes:
With the number of analyzed specimens (n), phylogenetic categories (PC), barcode index number (BIN), maximum intraspecific pairwise K2P distances (MID), minimum interspecific pairwise K2P distances to the nearest neighbour species (DNN), and the nearest neighbour species (NNS). Maximum intraspecific distances >2.2% and minimum interspecific distances <2.2% are marked in bold. At least one specimen of the compared species showed a distance value above or below this threshold in terms of a pairwise comparison. Asterisks (*) indicate species not recorded for Germany.