Figure 1.

Isoform analysis of wound-induced peroxidases of potato tubers. Total soluble proteins were extracted from either the (mechanically removed) suberized layer or the unsuberized tissue immediately below the suberized layer of 7-d wound-healed potato tubers, and chromatofocused on a Mono-P HR 5/5 column (Pharmacia). Proteins were loaded at pH 7.1, and the pH gradient (7.1–3.5 over approximately 15 min) started after all of the unbound protein had been washed through (indicated by an arrow). Fractions were assayed separately for activity using both ferulic acid and guaiacol.