Table I.
Purification summary for the anionic peroxidase isolated from potato tubers during wound healing
| Purification Step | Total Protein | Total Activitya | Purification | Recovery | RZbValue | |
|---|---|---|---|---|---|---|
| mg | μkat | μkat/mg protein | fold | % | ||
| Crude | 2,552 | 12,731 | 5 | 1 | 100.0 | NDc |
| AS pelletd | 790 | 8,578 | 11 | 2.2 | 67.4 | ND |
| SG 100 | 108 | 4,775 | 44 | 8.9 | 37.5 | 0.4 |
| PBE | 11.2 | 2,803 | 250 | 50.2 | 22.0 | 1.6 |
| DEAE | 3.5 | 2,517 | 719 | 144.2 | 19.8 | 2.7 |
Anionic peroxidase was purified from potato tubers 7 d after wounding.
a
Using ferulic acid as a substrate.
b
RZ, Reinheitszahl value (A405/A280) in deionized water.
c
ND, Not determined.
d
50% to 90% ammonium sulfate pellet.