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. Author manuscript; available in PMC: 2019 Mar 20.
Published in final edited form as: Acc Chem Res. 2018 Feb 16;51(3):736–744. doi: 10.1021/acs.accounts.7b00593

Figure 5. Epitope regions of IL-23 identified by FPOP, HDX-MS, and alanine shave mutagenesis.

Figure 5

(A) Critical peptides discovered by HDX (top) and FPOP (bottom). These regions are mapped on the linear sequence of IL-23 (in orange for HDX results and blue for FPOP). (B) Critical residues identified by FPOP (top) and alanine-shave mutagenesis (bottom) mapped on the IL-23 crystal structure. Amino acids identified by FPOP are in blue, and by mutagenesis in green. Gray regions suggest no significant difference in the conformation of IL-23 upon binding with Fab. Adapted with permission from ref. 9. Copyright 2017 American Chemical Society.