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. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2018 Apr 12;1865(7):959–969. doi: 10.1016/j.bbamcr.2018.04.005

Figure 4. Suppression of LGALS3 by shRNA in MSC reduces MYC expression and suppresses activation of AKT.

Figure 4

Protein lysates from control MSC (LKO) or MSC expressing LGALS3 shRNA were subject to electrophoresis and immunblot analysis performed using 2 separate filters. (A) Antibodies against Tubulin, LGALS3, MYC, p-AKT (S473), and total AKT were used. (B) Tubulin and ITGB3 antibodies were used. Densitometry using LiCor software was performed and ratio of protein relative to Tubulin assessed relative to LKO MSC are listed. Results for both (A) and (B) are representative of a single experiment that was performed three different times.