Skip to main content
. Author manuscript; available in PMC: 2019 Jul 1.
Published in final edited form as: Biochim Biophys Acta. 2018 Apr 12;1865(7):959–969. doi: 10.1016/j.bbamcr.2018.04.005

Table 1. List of antibodies used for RPPA.

MSC RPPA Staining Details
Hugo name Target Manufacturer Catalog# Antibody Source Antibody Dilution
AKT1 AKT1 Cell Signaling 2967 Mouse 300
AKT1/2/3 AKT1/2/3 Cell Signaling 9272 Rabbit 150
AKT1/AKT2/AKT3-phospho ser473 AKT-p473(Ser) Cell Signaling 9271 Rabbit 50
AKT1/AKT2/AKT3-phospho Thr308 AKT-p308(Thr) Cell Signaling 9275 Rabbit 50
AKT1S1 PRAS40 Invitrogen AHO103 1 Mouse 300
AKT1S1.p p-PRAS40 (Thr246) Cell Signaling 2997 Rabbit 200
AKT2 AKT2 Cell Signaling 2962 Rabbit 500
AKT3 AKT3 Cell Signaling 4059 Rabbit 200
NOL3 ARC (NOL3) Imgenex IMG-171 Rabbit 2000
ATF3 ATF3 Abcam ab87213 Rabbit 500
BAD Bad Cell Signaling 9292 Rabbit 100
BAD-phospho Ser112 Bad-p112(Ser) Cell Signaling 9291 Rabbit 100
BAD-phospho Ser136 Bad-p136(Ser) Cell Signaling 9295 Rabbit 50
BAD-phospho Ser155 Bad-p155(Ser) Cell Signaling 9297 Rabbit 100
BAK1 Bak Cell Signaling 3792 Rabbit 50
BAK1 Bak Abcam ab32371 Rabbit 50
BAX Bax Cell Signaling 2772 Rabbit 100
BCL2 Bcl2 DAKO M0887 Mouse 200
BCL2L1 Bcl-XL Cell Signaling 2762 Rabbit 500
BCL2L11 Bim Epitomics 1036-1 Rabbit 200
BECN1 Beclin-1 Cell Signaling 3738 Rabbit 500
BID Bid Cell Signaling 2002 Rabbit 250
BIRC5 Survivin Cell Signaling 2802 Rabbit 50
CAV1 Caveolin-1 Cell Signaling 3238 Rabbit 100
CCNB1 Cyclin B1 Santa Cruz SC245 Mouse 100
CCNB1 Cyclin B1 Epitomics 1495-1 Rabbit 1000
CCND1 Cyclin D1(M-20) Santa Cruz sc718 Rabbit 500
CCND3 Cyclin D3 Cell Signaling 2936 Mouse 100
CCNE1 Cyclin E Santa Cruz sc-247 Rabbit 100
CD34 CD34 Epitomics 2150-1 Rabbit 500
CDK1 CDC2 Calbiochem cc01 Mouse 200
CDK2 CDK2 Santa Cruz SC6248 Mouse 200
CDK4 CDK4 Cell Signaling 2906 Mouse 200
CDKN1A P21/Waf Cell Signaling 2946 Mouse 250
CDKN1A P21/Waf Santa Cruz sc-397 Rabbit 200
CDKN1B P27 Epitomic 1591-1 Rabbit 100
CDKN1B-pS10 P27-phosphoSer10 Epitomic 2187-1 Rabbit 500
PTEN PTEN Cell Signaling 9552 Rabbit 400
CREB1 phospho Ser133 CREB-p(ser133) Epitomics 1113-1 Rabbit 2000
CSNK2A1 CK2α Cell Signaling 2656 Rabbit 100
CTNNA1 Catenin-alpha Calbiochem CA1030 Mouse 75
CTNNB1 Catenin-beta Cell Signaling 9562 Rabbit 50
CTNNB1-phospho Ser33/37/Thr41 catenin-beta phospho- Cell Signaling 9561 Rabbit 500
DIABLO Smac/Diablo Cell Signaling 2954 Mouse 500
EGFR EGFR Santa Cruz sc-03 Rabbit 500
EGFR-phospho Tyr992 EGFR-p tyr992 Cell Signaling 2235 Rabbit 50
EGLN1 Egln1 Millipore 05-1327 Mouse 500
EIF2S1 elF2 Cell Signaling 9722 Rabbit 3000
EIF2S1-phospho Ser51 phospho-eIF2-alpha Cell Signaling 9721 Rabbit 250
EIF4E eIF4E Cell Signaling 9742 Rabbit 200
ELK1-phospho Ser383 ElK(phospho-ser383) Cell Signaling 9181 Rabbit 100
ERBB2 HER2/Erb2 Cell Signaling 2242 Rabbit 250
ERBB2 HER2/Erb2 Lab Vision (Fisher) MS-325-P0(MS32 5P0) Mouse 1000
ERBB2-phospho Tyr1248 HER2(p-Tyr1248) Upstate 06-229 Rabbit 1500
ERG ERG1/2/3 Santa Cruz sc-353 Rabbit 1000
FN1 Fibronectin Epitomics 1574 Rabbit 30000
FOXO1-phospho thr24/FOXO3-phospho thr32 FoxO1a/3a Cell Signaling 9464 Rabbit 500
FOXO3 FoxO3a Cell Signaling 9467 Rabbit 500
FOXO3 FoxO3a Cell signaling 2497 Rabbit 50
FOXO3-phospho Ser318/321 FKHRL1/FoxO 3a (P-Ser 318/321) Cell Signaling 9465 Rabbit 10000
GAB2 Gab2 Cell Signaling 3239 Rabbit 500
GAB2-phospho Tyr452 Gab2-pTyr452 Cell Signaling 3882 Rabbit 100
GAPDH GAPDH Ambion AM4300 Mouse 2000
GATA3 Gata3 BD bioscience 558686 Mouse 500
GSKA/GSKB GSK3 Santa Cruz sc-7291 Mouse 200
GSKA/GSKB-phospho Ser21/9 GSK3a/B(p-ser21/9) Cell Signaling 9331 Rabbit 200
HDAC3 HDAC3 Cell Signaling 2632 Rabbit 100
HIF1A HIF-1α BD Pharmingen 610959 Mouse 50
HNRNPK hnRNPK Santa Cruz sc-28380 Mouse 5000
HSP90AA1/HSP90AB1 HSP90 Cell Signaling 4875 Rabbit 500
HSPB1 HSP27 Cell Signaling 2402 Mouse 100
INPP5D SHIP1 Santa Cruz SC-8425 Mouse 250
IRS1-p1101 (serine) IRS1-phospho ser1101 Cell Signaling 2385 Rabbit 250
ITGA2 CD49b BD Transductio n Lab 611016 Mouse 500
ITGAL CD11a BD Transduction Lab 610826 Mouse 500
ITGB3 Integrin-beta3 Cell Signaling 4702 Rabbit 250
JMJD6 JMJD6 Abcam ab50720 Rabbit 1000
JUN-phospho Ser73 Jun-C-phospho ser73 Cell Signaling 9164 Rabbit 100
JUNB Jun-B Cell Signaling 3755 Rabbit 100
KDR VEGFR2 Cell Signaling 2479 Rabbit 700
KIT Kit-C Epitomics 1522 Rabbit 1000
LCK Lck Cell Signaling 2752 Rabbit 50
LEF1 LEF1 Cell Signaling 2230 Rabbit 1000
LGALS3 Galectin 3 Santa Cruz sc-32790 Mouse 250
LYN Lyn Cell Signaling 2732 Rabbit 250
MAP2K1 MEK Cell Signaling 9122 Rabbit 2000
MAP2K1/MAP2K2-phospho ser217/221 MEK(p-ser217/221) Cell Signaling 9121 Rabbit 1000
MAPK1 Erk2 Santa Cruz Sc-154 Rabbit 2000
MAPK1/MAPK3-phospho Thr202/Tyr204 Erk-p42/44(Thr202/ Tyr204 Cell Signaling 9101 Rabbit 400
MAPK14 P38 Cell Signaling 9212 Rabbit 200
MAPK14-phospho Thr180/tyr182 P38-pThr180/Tyr18 2 Cell Signaling 9211 Rabbit 100
MAPK8 JNK1/3 Santa Cruz sc-474 Rabbit 1000
MAPK9 JNK2 Cell Signaling 4672 Rabbit 25
MCL1 MCL1 BD pharmingen 559027 Mouse 50
MDM2 MDM2 Santa Cruz sc813 Rabbit 5000
MS4A1 I can't find the antigen name
MSI2 MSI2 Chemicon MAB100 85 Mouse 500
MTOR mTor Cell Signaling 2983 Rabbit 200
MTOR-phospho Ser2448 mTor(p-Ser2448) Cell Signaling 2971 Rabbit 100
MYC Myc Cell Signaling 9402 Rabbit 100
NOTCH1 cleaved val1744 Notch1-cleaved (Val1744) Cell Signaling 4147 Rabbit 400
NOTCH3 Notch3 Santa Cruz sc5593 Rabbit 200
NPM1 NPM Invitrogen 32-5200 Mouse 10000
NR4A1 Nur77 Imgenex IMG-528 Rabbit 200
NRP1 NRP1(neuropili n) Santa Cruz SC-5307 Mouse 10
PA2G4.pS65 4EBP1-phosph Ser65 Cell Signaling 9456 Rabbit 400
PA2G4.pT37_46 4EBP1-phospho Thr37/46 Cell Signaling 9459 Rabbit 20000
PA2G4.pT70 4EBP1-phospho Thr70 Cell Signaling 9455 Rabbit 400
PARK7 DJ-1 Private-Andreeff Lab in house antibody Rabbit 500
PARP1 PARP Cell Signaling 9542 Rabbit 200
PDK1 PDK1 Cell Signaling 3062 Rabbit 200
PDK1-phospho ser241 PDK1-p241(Ser) Cell Signaling 3061 Rabbit 500
PECAM1 CD31/PECAM DAKO M0823 Mouse 200
PPARG PPARγ Santa Cruz sc7273 Mouse 75
PPP2R2A/PP2R2B/PPP2R2 C/PPP2R2D PP2A-B55 Santa Cruz sc-18330 Goat 500
PRKAA1/PRKAA2 AMPKα Cell Signaling 2532 Rabbit 200
PRKAA1/PRKAA2-phospho Thr172 AMPKα P(Thr172) Cell Signaling 2535 Rabbit 200
PTEN PTEN Cell Signaling 9552
PTGS2 cox-2 Epitomics 2169-1 Rabbit 250
PTGS2 cox-2 Cell Signaling 4842 Rabbit 100
PTK2 Fak Cell Signaling 3285 Rabbit 500
RAC1/RAC2/RAC3 Rac1/2/3 Cell Signaling 2465 Rabbit 500
RELA NF-kB p65 Cell Signaling 3034 Rabbit 500
RPS6 S6 Ribosomal protein Cell Signaling 2217 Rabbit 250
RPS6-phospho ser235/236 S6 Ribosomal protein(phospho-ser235/236) Cell Signaling 2211 Rabbit 1500-2000
RPS6-phospho ser240/244 S6 Ribosomal protein(phospho-ser240/244) Cell Signaling 2215 Rabbit 750-1000
RPS6KB1 p70S6K Cell Signaling 9202 Rabbit 250
RPS6KB1 p70S6K Epitomics/a bcam 1494-1(ab3252 9)
RPS6KB1-phospho thr389 p70S6K(p-thr389) Cell Signaling 9205 Rabbit 250
SFN X14.3.3Sigma Upstate 05-632 mouse 200
SIRT1 SIRT1 Abcam ab32441 Rabbit 1000
SMAD1 smad1 Epitomics 1649-1 Rabbit 200
SMAD4 smad4 Santa Cruz sc7966 Mouse 1000
SMAD6 Smad6 Cell Signaling 9519 Rabbit 100
SPP1 Osteopotin Santa Cruz sc-21742 Mouse 500
SQSTM1 P62 Santa Cruz sc-28359 Mouse 250
SRC Src Upstate 05-184 Mouse 600
SRC-phospho tyr416 Src(phosphotyr416) Cell Signaling 2101 Rabbit 400
SRC-phospho tyr527 Src(phosphotyr527) Cell Signaling 2105 Rabbit 100
STAT1 stat1 Cell Signaling 9172 Rabbit 250
STAT1-phospho tyr701 Stat1(phosphotyr701) Cell Signaling 9171 Rabbit 100
STAT3 Stat3 Upstate 06-596 Rabbit 50
STAT3-phospho ser727 Stat3-p727(Ser) Cell Signaling 9134 Rabbit 100
STAT3-phospho tyr705 Stat3 p705(Tyr) Cell Signaling 9131 Rabbit 500
STAT5A/STAT5B Stat5 Cell Signaling 9352 Rabbit 250
STAT5A/STAT5B phospho Tyr694 Stat5(phospho-Tyr694) Cell Signaling 9351 Rabbit 100
STK11 LKB1/STK11 Cell Signaling 3050 Rabbit 500
TCF4 TCF-4 Santa Cruz sc8632 Goat 400
TGM2 TG2 Abcam ab2386 Mouse 2000
TP53 TP53 Cell Signaling 9282 Rabbit 1000
TP53-phosphoS15 TP53-phosphoS15 Cell Signaling 9284 Rabbit 200
TSC2 TSC2 Epitomic 1613-1 Rabbit 500
VHL VHL Novus NB100-485 Rabbit 2000
XIAP XIAP Cell Signaling 2042 Rabbit 100
YWHAE X14.3.3Epsilon Santa Cruz sc-23957 Mouse 200
YWHAZ 14-3-3-zeta Chemicon AB9746 Rabbit 750