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. Author manuscript; available in PMC: 2018 Oct 23.
Published in final edited form as: Nat Struct Mol Biol. 2018 Apr 23;25(5):372–383. doi: 10.1038/s41594-018-0056-2

Figure 2. New facultative heterochromatin domains form at low temperature.

Figure 2

(a) Genome-wide H3K9me relative fold enrichment in WT cells grown at 30°C or 18°C was determined by chromatin immunoprecipitation followed by microarray hybridization (ChIP-chip). Representative heterochromatin islands are indicated on the 30°C plot and new 18°C facultative heterochromatin peaks are indicated on the 18°C plot. (b) H3K9me relative enrichment at individual loci in WT cells grown at 30°C or 18°C, as determined by ChIP-chip. (c) H3K9me enrichments in WT cells grown at 30°C or 18°C for 1, 2, or 3 days were determined by ChIP-qPCR. H3K9me fold enrichments at SPAPB1A11.02, SPBC428.10, and SPBC1289.14 are shown relative to the control leu1 gene. Data presented is from a representative experiment performed at least twice. Source data for Fig. 2a are available with the paper online.