Figure 1.

Alignment of nucleotide sequences of α-tubulin cDNA clones. Sequences were aligned and displayed using the programs PileUp and Pretty (version 9.1, Genetics Computer Group, Madison, WI). Sequences homologous to the oligonucleotide primers used for PCR amplification are shown in lowercase; conserved nucleotides are indicated by solid black boxes; and dots indicate gaps that have been inserted for optimal alignment; the putative translational stop codons are marked by asterisks; and the restriction sites at which probe transcription was terminated are underlined. The restriction sites are cut by ScaI (GhTua1 and GhTua2), BsmAI (GhTua4), and SspI (GhTua5).