Table II.

α-Tubulin probes used for northern analysis

Probe	Template (Excluding Vector)	Nucleotide Identity
		GhTua1	GhTua2	GhTua3	GhTua4	GhTua5
		%
tua1UTR	GhTua1 (419–645)	100	46	37	45	39
tua2UTR	GhTua2 (416–755)	43	100	89	43	40
tua4UTR	GhTua4 (375–575)	48	41	48	100	38
tua5UTR	GhTua5 (404–587)	38	49	39	41	100
tua2ORF	GhTua2 (1–415)	88	100	99	83	78

α-³²P-labeled antisense RNA probes were transcribed from the cDNA clones (Fig. 1) following linearization with the appropriate restriction enzyme. Probes tua1UTR, tua2UTR, tua4UTR, and tua5UTR were transcribed from the 3′-UTR of the corresponding cDNA, whereas tua2ORF was transcribed from an ORF fragment. The nucleotide identity of the probes to each α-tubulin cDNA are shown. Values in parentheses are the corresponding nucleotide numbers.