Table 1.
Primer sequences used for gene expression
| Gene name | Primer sequence (5ˊ-3ˊ) | Annealing temperature(c) | Product size (bp) | Accession number |
|---|---|---|---|---|
| MTNR1A | F: TCGCCTCCATCCTC | 60 | 106 | XM _005698759.1 |
| R: AACACATTCCCTGCGT | ||||
| DNMT3b | F: GAAGATCCTACAAAGACAG | 60 | 115 | NM_18181302 |
| R: AATTTTCCCCTCCTTCTCCTGC | ||||
| DNMT1 | F: CGGAACTTCGTCTCCTTC | 60 | 114 | XM_015471996.1 |
| R: CACGCCGTACTGACCAG | ||||
| DNMT3a | F: AGCACAA CGGAGAAGCC | 60 | 192 | NM_001206502 |
| R: TTCCAGGAAGCAGTTCTTG | ||||
| YWHAZ | F: ATCTTGT GTCGTGTGGGG | 60 | 140 | XM_005689196.2 |
| R: CTCGG AGAACTTGCCATC | ||||