Extended Data Figure 6. Proteasome involvement in germline proteostasis.

a, LysoTracker-stained dissected germlines. b, GFP::PBS-1 localization. c, d, Schematic and imaging of proteasome sensor Ub^G76V::GFP. Active proteasomes degrade Ub^G76V::GFP, unless inhibited by MG132. e–g, GFP::RHO-1-aggregation following control or proteasomal pbs-1 RNAi. The gld-1(q485) mutation precluded aggregation following pbs-1 RNAi. This finding fits the model that the proteasome degrades GLD-1, but not the aggregates, consistent with aggregate engulfment by lysosomes. However, we note that proximal gld-1 germ cells, which form tumors²⁰, could potentially be non-permissive for aggregation. Mean ± s.d. from three biological replicates, each of n = 50 animals. ****P < 0.0001. Bars, 10 µm.