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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Comp Biochem Physiol C Toxicol Pharmacol. 2017 Nov 20;208:38–46. doi: 10.1016/j.cbpc.2017.11.003

Fig. 2. Agarose Array Embedding.

Fig. 2

To facilitate larval embedding, casting molds were used to create agarose array blocks. (A) Casting mold taped all the way around the sides before the addition of agarose. (B) Taped casting mold with the agarose added. (C) Agarose block and casting mold after removal of tape (inset: closeup of a corner showing the agarose on top and acrylic mold below it). (D) Closeup view of larvae in the “triangle mold” (E) “radius mold” and (F) “shoe mold” from above to illustrate larval position during embedding. A slight bending apparent for both “triangle” and “radius” molds is not present in the “shoe” mold closeup.