Figure 4. Raspberry does not increase BAT metabolic activity in AMPKα1−/− mice challenged with HFD.
AMPKα1flox/flox mice (WT) and RosaCre/AMPKα1flox/flox (AMPKα1−/−, α1KO) mice were fed with HFD with/without 5% raspberry for 10 weeks, and BAT were sampled for histochemical and biochemical analyses. A, representative H&E staining and UCP1 staining for BAT sections. B, mRNA expression of thermogenic genes in BAT. C, representative images of immunoblotting. D, contents of thermogenic proteins and AMPKα. *P < 0.05 and **P < 0.01. Data are expressed as means ± SEM (n = 6).
