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. 2018 Apr 25;8(4):180024. doi: 10.1098/rsob.180024

Figure 3.

Figure 3.

ER stress markers in AppNL-G-F and APP-Tg mice. (a) Western blot analyses of ER stress markers in the hippocampi of six-month-old (M) WT, AppNL-G-F, APP23 and Tg2576 mice. Expression in 6- and 15-month-old APP/PS1 and 23-month-old 3XTg-AD mice were also detected. Values shown in figures are the band intensity for each band which is normalized to β-actin values (for GRP78, CHOP and PDI) or total eIF2α (for p-eIF2α). As a positive control, ER stress markers in thapsigargin-treated primary cultured cortical neuronal cells or Neuro2a cells were confirmed. Arrowhead shows bands for CHOP, and asterisk shows non-specific bands. (b) Expression levels of ER stress markers were normalized to that of β-actin (for GRP78, CHOP and PDI) or total eIF2α level (for p-eIF2α), and reported as relative levels compared with expression in six-month-old WT mice. Data are shown as means ± s.e.m. (n = 3). Differences between groups were examined for statistical significance with one-way ANOVA. n.s.: no significant difference.