FIGURE 2.

Alzheimer disease (AD)-related CK2 activation induces SET phosphorylation and its cytoplasmic translocation. Primary neuronal culture were treated with Aβ (A) or AAV2- hTau (B) for 72 h. CK2 activity was detected by the CK2 kinase Assay/Inhibitor Screening kit CY-1170. The lysate from Aβ (C,G) or AAV2- hTau (E,G) treated primary neuronal culture was collected for Western blots using antibodies against SET, SET pS9 (phospho S9), STAT1, STAT1 pY701, β-actin and DM1A, n = 3. (D,F,H) Quantitative analysis of the blots in (C,E,G) respectively. Immunofluorescence showed subcellular localization and phosphorylation of SET (I) and STAT1 (J) in primary neuronal culture under treatment with Aβ and TBB, a CK2 inhibitor. Scale bar in (I,J), 150 μm; in insets, 25 μm, n = 4. All data represent mean ± SEM (t-test), ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001 vs. control (Ctr).