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. 2018 Apr 30;11:136. doi: 10.3389/fnmol.2018.00136

Figure 5.

Figure 5

CD200 knock-out NPCs are rejected more rapidly from the striatum. To study whether CD200 expression has a role in protecting striatal grafts from rejection, we transplanted to the striatum BrdU-labeled CD200KO NPCs or wt NPCs (n = 7 mice per group). Two months after transplantation, the CD200KO-NPCs (A) showed 40% lower survival compared to wt NPCs (D, P = 0.05, by student T test). Examination of the brain immune response revealed strong reaction of microglia/macrophages in both CD200KO and wt transplanted brains (B,C enlargement; E,F enlargement, respectively). However, T cell infiltration was significantly stronger in striata transplanted with CD200KO NPCs (arrows, B,C enlargement). The red auto-fluorescence represents dead grafted cells and was distinguished from CD3 expression in high power field microscopy. Str, striatum; LV, lateral ventricle. Scale Bar: (A,D) 0.5 mm, (B,E) 100 μm, (C,F) 20 μm.