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. 2018 Apr 30;9:151. doi: 10.3389/fgene.2018.00151

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Copyright © 2018 Symonenko, Roshina, Krementsova and Pasyukova.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PCR analysis of esg structure in revertant lines. PCR primers esg1 and esg2 were used with an expected product size of 508 bp. 1: marker (GeneRuler™ 100 bp Mass DNA Ladder, Fermentas); 2: esgP (negative control, an expected PCR product of approximately 9 kb is not produced under the PCR conditions used); 3: Control M; 4: Rev1.1; 5: Rev1.2; 6: Rev2.1; 7: Rev2.2; 8: Rev3; 9: Rev4; 10: Rev5.