Table 1.

Distributive statistics of life span.

Exp. no	Line	N	Mean	Standard error	Median	Percentile 90	P-values for comparisons with the corresponding control lines		Tukey test
							Mann-Witney test	Kolmogorov-Smirnov test
esg MUTATION, UNMATED MALES
1	Control	50	50	1.9	54	61			A
	esgP	50	61	2.1	63	77	<0.0001	<0.001	B
	rev3	49	57	2.8	52	77	0.7029	<0.005	A
	rev5	49	65	1.8	66	78	<0.0001	<0.001	B
2	Control	150	46	1.2	48	63			A
	esgP	150	70	1.6	79	86	<0.0001	<0.001	B
	rev3	150	44	0.7	44	51	0.0840	<0.005	A
3	Control M	100	36	1.4	38	53
	esgP	100	57	1.9	66	72	0.0001	<0.001
4	Control M	150	28	1.1	26	48			A
	esgP	150	55	1.3	58	71	0.0001	<0.001	B
	Control M × esgP	50	45	2.6	52	63	0.0006	<0.005	C
5	Control M	195	45	1.2	48	61			A
	esgP	110	66	1.5	69	83	0.0001	<0.001	B
	Rev3	150	49	1.4	48	76	0.1856	< 0.1	A
	Rev5	205	56	1.2	59	77	0.0001	<0.001	C
esg MUTATION, UNMATED FEMALES
1	Control M	50	62	2.1	67	77
	esgP	50	65	1.8	67	83	0.5533	>0.10
3	Control M	100	36	1.7	38	53
	esgP	100	48	1.6	55	65	0.0001	<0.001
4	Control M	150	41	1.2	45	59			A
	esgP	150	51	1.5	56	72	0.0001	<0.001	B
	Control M × esgP	50	49	2.2	54	63	0.0003	<0.001	C
5	Control M	114	45	1.5	47	66			A
	esgP	124	58	1.6	61	77	0.0001	<0.001	B
	Rev3	114	50	1.9	51	76	0.0243	< 0.1	A
	Rev5	115	56	1.3	54	72	0.0001	<0.001	B
esg MUTATION, MATED MALES
6	Control M	60	30	1.9	30	50
	esgP	60	46	2.2	50	66	0.0001	<0.001
7	Control M	60	33	1.5	36	45
	esgP	60	44	2.3	46	67	0.0001	<0.001
esg MUTATION, MATED FEMALES
6	Control M	60	37	1.6	38	50
	esgP	60	37	1.9	37	57	0.9581	>0.1
7	Control M	60	34	1.4	37	45
	esgP	60	34	1.9	34	50	0.8011	>0.1
esg RNA-i KNOCKDOWN IN THE NERVOUS SYSTEM, UNMATED MALES
8	Control K2	100	51	1.6	52	70
	Kdw2	100	47	0.9	46	59	0.0112	<0.005
	Kdw3	100	58	1.8	58	79	0.0021	<0.025
9	Control 2	100	61	1.5	64	74
	Kdw3	100	65	1.7	67	85	0.0351	<0.05
esg RNA-i KNOCKDOWN IN THE NERVOUS SYSTEM, UNMATED FEMALES
8	Control K2	100	76	2.0	82	97
	Kdw2	100	71	2.1	77	92	0.0111	< 0.1
	Kdw3	100	84	1.6	90	98	0.0004	<0.001
9	Control K2	100	88	1.4	92	101
	Kdw3	100	93	1.3	95	105	0.0049	<0.025

N is a number of flies in the experiment; Median indicates a number of days when 50% of flies are dead; Percentile 90 indicates a number of days when 90% of flies are dead. Standard errors were calculated from the variance among vials. Significant P-values are in bold case. When multiple lines were compared, an underscored line indicates significant P-values that did not survive Bonferroni correction. The mean life spans and survival curves of Control M and esgP flies from experiments 1 and 2 have already been published (Magwire et al., 2010). Control M, the w¹¹¹⁸(F) line where the P{GT1} insertion in the 3′ regulatory region of esg was obtained obtained (Magwire et al., 2010). esgP, the line with an esg^BG01042 mutation in the w¹¹¹⁸(F) background. Rev3, the line with the precise P{GT1} excision. Rev5, the line with the imprecise P{GT1} excision. Control K2, the y¹ v¹; P{y^+t7.7 = CaryP}attP2 line proposed as a control line for Kdw2 and Kdw3 by the manufacturer (http://flystocks.bio.indiana.edu/Browse/TRiPtb.htm). Kdw2, the y¹ v¹; P{TRiP.JF03134}attP2 line used to provide esg RNAi knockdown. Kdw3, the y¹ v¹; line used to provide esg RNAi knockdown.