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. 2018 Apr 30;11:142. doi: 10.3389/fnmol.2018.00142

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Copyright © 2018 Yamagata, Duan and Sanes.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cdh2 supports dendritic branches neurexin (NRXN)-dependent postsynaptic differentiation. (A,B) Rat hippocampal neurons were cultured with 293TA (A) or 293NC (B) cells transfected with NRXN, then stained with anti-MAP2 to visualize dendrites. 293 cells were visualized by phalloidin staining (green in A′,B′). (C,D) Immunostaining for PSD95 on NRXN-transfected 293TA (C) or 293NC cells (D). Bar, 20 μm. (E,F) Quantification of MAP2-positive protrusions (relative areas) (E) and PSD95 punta (F). F_(3,36) = 13, Pr(>F) = 8.2e-06 by one-way ANOVA followed by Tukey multiple pairwise-comparisons; *p < 0.0002.